Thermo Fisher Scientific Apolipoprotein B Monoclonal Antibody (6G6)

Thermo Fisher Scientific Apolipoprotein B Monoclonal Antibody (6G6)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA5-15851 targets ApoB in indirect ELISA, FACS, IF, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human ApoB expressed in E. coli.
MA5-15851 detects ApoB with a predicted molecular weight of approximately 516 kDa.

Target Information

Apolipoprotein B (ApoB) is a major protein component of low-density lipoproteins (LDL) in human plasma, with a molecular mass of approximately 260–500 kDa.
It binds to specific cell membrane receptors and plays a key role in the removal of LDL and very low-density lipoprotein (VLDL) cholesterol from circulation.

LDL acts as the carrier protein for cholesterol in the blood. It binds to LDL receptors on capillary walls, mediating cholesterol uptake and clearance. In atherosclerotic lesions, oxidatively modified LDL is found and recognized by macrophages via scavenger receptors A and CD36.

ApoB is primarily synthesized in the liver and serves as a major apolipoprotein of VLDL, IDL, and LDL, as well as a component of lipoprotein(a). Elevated ApoB levels are associated with premature atherosclerosis. Normal plasma ApoB levels are around 800 mg/L.

ApoB occurs in two main isoforms—ApoB-48 and ApoB-100—encoded by a single gene through RNA editing. Mutations in the ApoB gene or its regulatory regions can cause hypobetalipoproteinemia and hypercholesterolemia due to ligand-defective ApoB.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.