Thermo Fisher Scientific CD33 Monoclonal Antibody (WM53)

Thermo Fisher Scientific CD33 Monoclonal Antibody (WM53)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• For flow cytometry, use 10 µL of the suggested working dilution to label 10⁶ cells.
• Store product as a concentrated solution.
• Centrifuge briefly prior to opening the vial.

Target Information

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family.
It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules capable of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies.
These ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface.

CD33 is expressed on cells of myelomonocytic lineage and binds sialic acid residues in N- and O-glycans on cell surfaces.
It is a therapeutic target for acute myeloid leukemia and is found on granulocyte and macrophage precursors in the bone marrow, but not on pluripotent stem cells.
CD33 is also expressed on peripheral monocytes and serves as a useful marker for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias.
Diseases associated with CD33 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.