Thermo Fisher Scientific eIF3G/EIF3S4 Polyclonal Antibody
Rabbit polyclonal antibody targeting human eIF3G/EIF3S4. Validated for WB and IP applications. Predicted cross-reactivity with mouse, rat, and bovine. Supplied as a liquid, 0.20 mg/mL, stored at 4°C. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 175 and 225 of human eukaryotic translation initiation factor 3, subunit G (eukaryotic translation initiation factor 3, subunit 4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, and Bovine
Target Information
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC).
It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition.
The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
This subunit can bind 18S rRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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