Thermo Fisher Scientific Phospho-PAK1/PAK2/PAK3 (Thr423, Thr402, Thr421) Polyclonal Antibody
PAK1/PAK2/PAK3 단백질의 인산화 상태(Thr423, Thr402, Thr421)를 검출하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein A 정제, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of Thr423 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
In mammals, several isoforms of p21-Activated Protein Kinases (PAKs) have been identified.
- α-PAK (PAK1) and β-PAK (PAK3) are mostly brain-specific.
- γ-PAK (PAK2) is expressed ubiquitously (Jakobi et al., 2003).
Mutations in the PAK3 gene are associated with X-linked mental retardation. PAK3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). It also plays a role in regulating cell shape, motility, and cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 is essential for PAK activation (Jakobi et al., 2000).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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