Thermo Fisher Scientific Hexanoyl-Lysine adduct Monoclonal Antibody (5E8), FITC
Hexanoyl-Lysine adduct를 특이적으로 검출하는 FITC 결합 단일클론 항체로, WB, IHC, ICC, Flow Cytometry, ELISA에 사용 가능. Hexanoyl-Lysine 변형 단백질에만 반응하며, 다른 산화 부산물과 교차반응 없음. 연구용으로 사용.
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Thermo Fisher Scientific Hexanoyl-Lysine adduct Monoclonal Antibody (5E8), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:26 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5E8 |
| Immunogen | Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932236 |
Additional Formats
- Unconjugated (Cat. MA5-27555)
- APC (Cat. MA5-45781)
- PE (Cat. MA5-45784)
- PerCP (Cat. MA5-45783)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45782 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins.
Does not detect free Hexanoyl-Lysine.
Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Hexanoyl-lysine (HEL) is a novel lipid peroxidation biomarker derived from the oxidation of omega-6 unsaturated fatty acids.
HEL may be used as a biomarker in omega-6 PUFA (polyunsaturated fatty acid) oxidation in living organisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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