Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (6F10), APC
4-Hydroxy-2-hexenal 및 4-Hydroxynonenal 변형 단백질을 특이적으로 검출하는 마우스 단클론 항체. Western blot, ICC/IF, Flow cytometry, ELISA에 적합. APC 형광 표지로 651/660 nm에서 발광. 보존제 무첨가, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6F10 |
| Immunogen | Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932239 |
Available Formats
- Unconjugated (Cat. MA5-27556)
- FITC (Cat. MA5-45786)
- PE (Cat. MA5-45788)
- PerCP (Cat. MA5-45787)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45785 was sufficient for detection of 4-Hydroxy-2-hexenal in 0.5 µg of 4-Hydroxy-2-hexenal conjugated to BSA and 4-Hydroxy nonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins.
Does not detect free 4-HHE or 4-HNE.
Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal (4-HNE, C9H16O2) is an α,β-unsaturated hydroxyalkenal produced by lipid peroxidation in cells. It is the primary α,β-unsaturated hydroxyalkenal formed during this process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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