Thermo Fisher Scientific GTF2H2/BTF2/TFIIH Basal Transcription Factor Monoclonal Antibody (PCRP-GTF2H2-1B9)
인간 GTF2H2 단백질을 표적으로 하는 단클론 항체로, TFIIH 복합체 연구에 적합합니다. ICC/IF, Flow Cytometry, Peptide Array 등 다양한 응용 가능. 단백질 A/G로 정제된 액상 형태이며, 4°C 보관.
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Thermo Fisher Scientific GTF2H2/BTF2/TFIIH Basal Transcription Factor Monoclonal Antibody (PCRP-GTF2H2-1B9)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-GTF2H2-1B9 |
| Immunogen | Recombinant full-length human GTF2H2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Initiation of transcription from protein-coding genes in eukaryotes requires RNA polymerase II and several basal transcription factors. Binding of TFIID (TBP) to the TATA box initiates formation of a multiprotein complex including TFIIA, TFIIB, TFIIE, TFIIF, and TFIIH.
TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor with multiple enzymatic activities. Its core comprises five subunits: p89 (XPB/ERCC3), p62, p52, p44, and p34. Additional subunits include p80 (XPD/ERCC2) and the ternary kinase complex (Cdk7, cyclin H, MAT1).
Both p89 and p80 exhibit ATP-dependent helicase activity, while p62, p52, and p44 are involved in nucleotide excision repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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