Thermo Fisher Scientific pan Cytokeratin (pan CK) Monoclonal Antibody (AE1+AE3), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:150

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AE1+AE3

Immunogen

Human epidermal cytokeratins if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P08727-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:400,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P08727-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P08727-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:Q04695-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:432,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_Q04695-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_Q04695-1_House_mouse_PDP.jpeg,sortOrder:10},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P08779-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:473,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P08779-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P08779-1_House_mouse_PDP.jpeg,sortOrder:11},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P19012-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:456,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P19012-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P19012-1_House_mouse_PDP.jpeg,sortOrder:12},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P04264-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:644,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P04264-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P04264-1_House_mouse_PDP.jpeg,sortOrder:13},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P08729-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:469,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P08729-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P08729-1_House_mouse_PDP.jpeg,sortOrder:14},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P12035-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:628,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P12035-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P12035-1_House_mouse_PDP.jpeg,sortOrder:15},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P35908-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:639,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P35908-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P35908-1_House_mouse_PDP.jpeg,sortOrder:16},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P02533-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:472,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P02533-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P02533-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P13647-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:590,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P13647-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P13647-1_House_mouse_PDP.jpeg,sortOrder:3},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P13645-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:584,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P13645-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P13645-1_House_mouse_PDP.jpeg,sortOrder:4},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P05783-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:430,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P05783-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P05783-1_House_mouse_PDP.jpeg,sortOrder:5},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P05787-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:483,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P05787-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P05787-1_House_mouse_PDP.jpeg,sortOrder:6},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P13646-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:458,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P13646-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P13646-1_House_mouse_PDP.jpeg,sortOrder:7},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P19013-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:520,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P19013-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P19013-1_House_mouse_PDP.jpeg,sortOrder:8},{targetFamily:Cytokeratin Pan Type I/II,uniProtId:P02538-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:564,antigenImageFileName:CF190321_Cytokeratin_Pan_Type_III_P02538-1_House_mouse.svg,antigenImageFileNamePDP:CF190321_Cytokeratin_Pan_Type_III_P02538-1_House_mouse_PDP.jpeg,sortOrder:9}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.