Thermo Fisher Scientific p53 (Tumor Suppressor Protein Monoclonal Antibody (PCRP-TP53-2A10)

Applications and Tested Dilution

Product Specifications

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. Upon activation, p53 induces transcription of genes such as p21^waf1^, leading to G1 or G2/M arrest.

Structurally, p53 includes:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerization domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is crucial for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 levels are typically low in normal cells but elevated in transformed cells, contributing to malignancy. Mutations in p53 often result in loss of DNA-binding and tumor-suppressing functions. Germline mutations in TP53 are linked to cancer-prone syndromes such as Li-Fraumeni.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.