Thermo Fisher Scientific Lymphocyte Activation Gene 3 (LAG3) Monoclonal Antibody (OTI7E3)

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Flow Cytometry (Flow)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. It binds MHC class II with higher affinity than CD4 and is involved in the negative regulation of T cell activation and homeostatic proliferation.
LAG-3 expression is observed on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells typically express higher levels of LAG-3 compared to CD4+ T cells.
Coexpression of LAG-3 and CD49b identifies human and mouse Type 1 regulatory T cells (Tr1 cells).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.