Thermo Fisher Scientific Lamin B2 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Lamin B2 for WB, IHC, and ICC applications. High specificity with antigen affinity purification. Supplied as liquid, 1 mg/mL, unconjugated form. Suitable for U87-MG positive control; store at -20°C for long-te...
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | — |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 277–560 of human Lamin B2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA and 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546597 |
Product Specific Information
- Recommended positive control: U87-MG
- Predicted reactivity: Bovine (83%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic side of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type and B-type lamins.
A-type lamins include lamin A, lamin C, and lamin Adel 10, all arising from the same gene via alternative splicing.
B-type lamins include lamin B1 and lamin B2, encoded by distinct genes.
Mutations in A-type lamins are linked to rare dominant genetic disorders such as Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease, and Dunnigan-type familial partial lipodystrophy.
Expression of A-type lamins correlates with cell differentiation and chromatin interaction, suggesting a role in regulating differential gene expression.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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