Thermo Fisher Scientific c-Jun Recombinant Superclonal Antibody (2HCLC)
Western blot, ICC/IF, Flow cytometry, ChIP 등 다양한 응용에 적합한 c-Jun 항체. 인간 및 생쥐 반응성, Expi293 발현 재조합 Superclonal 항체로 높은 특이성과 재현성 제공. 단백질 A 정제, 액상형, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:250–1:2,500 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | – |
| Flow Cytometry (Flow) | – | View 1 publication |
| ChIP assay (ChIP) | 2.5 µg × 10⁶ cells | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Antibody |
| Clone | 2HCLC |
| Immunogen | Peptides corresponding to Human JUN (aa 21–36, 211–228) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2633131 |
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Pig, and Mouse.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They combine multiple recombinant monoclonal antibodies, providing the sensitivity of polyclonal antibodies and the specificity and consistency of monoclonal antibodies.
These antibodies recognize multiple epitope sites, enhancing detection sensitivity for low-abundance targets. Each lot contains a known mixture of light and heavy chains, ensuring reproducibility and eliminating biological variability typical of polyclonal production.
Note: Formerly called “Recombinant polyclonal antibody”, now rebranded as “Recombinant Superclonal™ antibody”. The product and performance remain unchanged.
Target Information
c-Jun is a transcription factor that binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes NR5A1 activity when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon cAMP pathway stimulation. It is also involved in KRAS-mediated transcriptional activation of USP28 in colorectal cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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