Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (236-10501)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 68 publications 68 publications

Immunohistochemistry (IHC)

-

View 17 publications 17 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:500

View 64 publications 64 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 14 publications 14 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse

Published species

Bovine, Fruit fly, Hamster, Human, Mouse, Non-human primate, Pig, Plant, Rat, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

236-10501

Immunogen

Bovine Alpha-Tubulin.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Lyophilized

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_10563441

Product Specific Information

The anti-bovine alpha-tubulin antibody can be used with secondary detection techniques to visualize microtubules in fixed cells and in fixed or frozen tissue sections of various species. In addition, this antibody can be used to detect tubulin by ELISA and immunoblot, for the screening of expression libraries and, since it recognizes amino acids 65-97 of the N-terminal structural domain, as a probe for the N-terminal domain of alpha-tubulin.

We have found that this highly specific antibody can be used at much lower concentrations than anti-tubulin antibodies available from other suppliers. For immunocytochemistry applications, we recommend using an initial concentration of 1 µg/mL or less.

Dissolve in PBS with 1% BSA and 2 mM sodium azide to obtain a stock concentration of 0.2 mg/mL.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.