Thermo Fisher Scientific PUMA alpha Polyclonal Antibody
PUMA 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 친화 크로마토그래피로 정제되었습니다. 단기 4°C, 장기 -20°C 보관 권장. 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): 0.1–0.5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PUMA (145–159aa ADDLNAQYERRRQEE) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide, 0.05 mg thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2884862 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
PUMA is a pro-apoptotic Bcl-2 family member, along with Bax and Noxa, which are transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins, PUMA-a and PUMA-b. These proteins bind Bcl-2, localize to mitochondria, and induce cytochrome c release and apoptosis in response to p53 activation. PUMA likely mediates p53-induced apoptosis via the cytochrome c/Apaf-1-dependent pathway. Antisense inhibition of PUMA expression reduces the apoptotic response to p53.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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