Thermo Fisher Scientific IDH2 Polyclonal Antibody
Thermo Fisher Scientific의 IDH2 Polyclonal Antibody는 인간 및 마우스 반응성을 가진 rabbit IgG 항체로, WB, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific IDH2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,500 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 402–452 of human Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For ICC: formaldehyde fixation and Triton-X 100 permeabilization recommended
- For IHC (FFPE tissue): epitope retrieval with citrate buffer pH 6.0 recommended
- Predicted to react with Pig (based on 100% sequence identity)
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes are classified into two subclasses: NAD-dependent and NADP-dependent. Five isocitrate dehydrogenases have been reported—three NAD-dependent (mitochondrial matrix-localized) and two NADP-dependent (one mitochondrial, one cytosolic). The NADP-dependent mitochondrial isozyme encoded by this gene plays a role in intermediary metabolism and energy production and may interact with the pyruvate dehydrogenase complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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