Thermo Fisher Scientific Phospho-AMPK alpha-1 (Ser496) Polyclonal Antibody
Phospho-AMPK alpha-1 (Ser496) 폴리클로날 항체로 Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. Rabbit IgG 호스트, 액상 형태, 1 mg/mL 농도. 연구용으로 세포 신호전달 및 인산화 단백질 분석에 적합.
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Recommended Positive Control |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | HUVEC cells |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | Human colon carcinoma |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 | HeLa cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 496 (S-G-SP-V-S) derived from Human AMPK-alpha-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554298 |
Product Specific Information
A suggested positive control for Western blot is HUVEC cells; suggested positive control for IHC is human colon carcinoma; suggested positive control for ICC/IF is HeLa cells.
Target Information
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein. Through this mechanism, protein kinases mediate most signal transduction in eukaryotic cells, regulating metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation.
With more than 500 gene products, the protein kinase family is one of the largest families in eukaryotes, classified into eight major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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