Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Polyclonal Antibody
Phospho-Histone H3 (Ser10) 특이적 폴리클로날 항체로, Western blot, ICC/IF, IP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Rabbit IgG 호스트 기반, 항원 친화 크로마토그래피로 정제. 세포핵 내 히스톤 변형 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide containing -QTARKSTGGKA- with phosphorylated serine 10 of human Histone H3 (amino acid range 6–16). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: 3T3 Nuclear Extract
- Cellular location: Nucleus
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it binds to DNA to form nucleosomes. Histones are essential for transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 undergoes various post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and transcription factor binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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