Thermo Fisher Scientific CD5 Monoclonal Antibody (UCHT2), APC-eFluor 780, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 2 publications

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by most thymocytes, mature T cells, and a subset of B cells. CD5 signaling activates T cells and mediates T-B interactions through binding to CD72 on B cells. The UCHT2 clone recognizes primate CD5.

Applications Reported:
Validated for use in flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 5 µL (0.25 µg) per test (in 100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:

• Excitation: 633–647 nm (Red Laser)
• Emission: 780 nm
• Light-sensitive tandem dye – protect from light.

Fixation Guidance:
Samples can be stored in:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-57)
  Storage up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Target Information

CD5 is a 67 kDa human T-lymphocyte transmembrane glycoprotein expressed on all mature T cells, most thymocytes, and some B cells. It is involved in regulating T-cell proliferation and T-B interactions. CD5 is found on ~95% of thymocytes and ~72% of peripheral blood lymphocytes, with predominant expression in T-cell areas of lymph nodes.
It is associated with T-cell leukemias, lymphomas, thymus cancer, and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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