Thermo Fisher Scientific NFIA Polyclonal Antibody
Rabbit polyclonal antibody against human NFIA 단백질. IHC(P)와 ICC/IF에 적합하며, 항원 친화 크로마토그래피로 정제됨. PBS/glycerol buffer에 보존되고, 단기 4°C, 장기 -20°C 보관 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NFIA. Recombinant protein control fragment (Product #RP-89382) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644644 |
Product Specific Information
Immunogen sequence:
VKSPQCSNPG LCVQPHHIGV SVKELDLYLA YFVHAADSSQ SESPSQPSDA DIKDQPENGH LGFQDSFVTS GVFSVTELVR VSQTPIAAGT GPNFSLSDLE SSSYYSMSPG A
Antigen sequence identity:
- Mouse: 99%
- Rat: 100%
Target Information
NF-1, also designated CTF, is a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Two forms of NF-1 arise from alternative splicing of a single gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain and activates transcription through a proline-rich carboxy-terminal transactivation domain. It recognizes and binds the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element is found in promoter regions of various eukaryotic genes, including Ha-Ras, alpha-globin, HSP70, GRP78, Histone H1, myelin basic protein, and Xenopus laevis vitellogenin gene promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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