Thermo Fisher Scientific IL12A Monoclonal Antibody (OTI2A8), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Interleukin 12A (IL12A) is a signaling cytokine belonging to the Interleukin-6 superfamily.
The IL-12 alpha chain precursor (IL-12A) forms a 35-kDa subunit of the disulfide-linked IL-12 heterodimer and exhibits diverse biological activities.
IL-12A acts as a growth factor for activated T and NK cells, enhancing NK/lymphokine-activated killer cell lytic activity and stimulating IFN-γ production.
It promotes differentiation of Th1 and Th2 cells and regulates tyrosine phosphorylation of Stat4 protein.
IL-12A signaling in innate immunity requires nitric oxide synthase 2A.
In combination with EBI3, IL-12A forms the heterodimeric cytokine IL-35, produced by Treg cells and essential for maximal suppressive activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.