Thermo Fisher Scientific H5N1 VN04-13 Monoclonal Antibody (14C5)
H5N1 인플루엔자 A 바이러스 HA 단백질에 특이적인 마우스 모노클로날 항체로, ELISA, WB, IHC, IP 등 다양한 분석에 사용 가능. 교차 반응 없음. 단백질 A로 정제된 액상 형태로 -20°C에서 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:5,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14C5 |
| Immunogen | Concentrated purified virus preparation containing hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Specific for H5 hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)]. VN04-13 monoclonal antibody did not cross-react with influenza viruses of other HA subtypes. This monoclonal antibody reacted with H5N1 influenza viruses representatives of different clades and subclades of the H5 HA subtype.
Target Information
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic.
Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication.
The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin. The distinct antigenic properties of the new swine influenza virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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