Thermo Fisher Scientific GAPDH Monoclonal Antibody (33F2.C8.G6.E3)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot contents and freeze at -20°C or below for extended storage.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Anti-GAPDH Monoclonal Antibody is directed against rabbit Glyceraldehyde-3-Phosphate Dehydrogenase.
This antibody is expected to react with rabbit, human, and mouse based on 100% sequence homology.
Reactivity against other species is not known.

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis.
It exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.

Beyond glycolysis, GAPDH participates in:

• DNA replication and repair
• Nuclear RNA export
• Membrane fusion and microtubule bundling
• Gene expression regulation during apoptosis
• Cellular processes related to neurodegenerative diseases (e.g., Alzheimer’s, Huntington’s)

Due to its high expression in most tissues, GAPDH is widely used as a protein loading control in Western blot analysis.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.