Thermo Fisher Scientific GAPDH Monoclonal Antibody (33F2.C8.G6.E3)
GAPDH 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rabbit 반응성. 1 mg/mL 액상 형태이며 -20°C 보관. 세포 단백질 발현 분석 및 로딩 컨트롤에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rabbit |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 33F2.C8.G6.E3 |
| Immunogen | Anti-GAPDH Monoclonal Antibody was produced by repeated immunizations in mice with rabbit GAPDH protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | 0.1M Tris HCl/TBS, pH 8 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-GAPDH Monoclonal Antibody is directed against rabbit Glyceraldehyde-3-Phosphate Dehydrogenase.
This antibody is expected to react with rabbit, human, and mouse based on 100% sequence homology.
Reactivity against other species is not known.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis.
It exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in:
- DNA replication and repair
- Nuclear RNA export
- Membrane fusion and microtubule bundling
- Gene expression regulation during apoptosis
- Cellular processes related to neurodegenerative diseases (e.g., Alzheimer’s, Huntington’s)
Due to its high expression in most tissues, GAPDH is widely used as a protein loading control in Western blot analysis.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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