Thermo Fisher Scientific AKAP8 Polyclonal Antibody
AKAP8 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 액상 형태로 제공됩니다. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human AKAP8 (Accession O43823), corresponding to amino acid residues E343–S393 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2850507 |
Product Specific Information
This antibody detects endogenous levels of total AKAP8.
Target Information
The type II cAMP-dependent protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by compartmentalization of the kinase to specific sites within the cell. The R subunit (RII) of PKA interacts with specific RII-anchoring proteins, known as A-kinase anchoring proteins (AKAPs).
AKAP95, also known as AKAP8, is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney, and skeletal muscle. During mitosis, AKAP95 is recruited to chromosomes and plays an essential role in mitotic progression. It participates in PKA signaling through interaction with the RII regulatory subunit and forms a complex with HA95 and HDAC3, which is required for histone H3 deacetylation during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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