Thermo Fisher Scientific BMI-1 Polyclonal Antibody
BMI-1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, ICC, IP 등 다양한 응용에 적합합니다. Human 및 Mouse에 반응하며 고순도의 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–273 of Human BMI1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547365 |
Product Specific Information
- Recommended positive controls: 293T, A549, H1299, HCT116, K562, THP-1, HL-60, C8D30, BCL-1, Raw264.7
- Predicted reactivity: Mouse (97%), Rat (93%), Xenopus laevis (91%), Dog (99%), Cat (99%), Pig (100%), Chicken (97%), Chimpanzee (99%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Component of the Polycomb group (PcG) multiprotein PRC1 complex, which maintains the transcriptionally repressive state of many genes, including Hox genes, throughout development.
The PRC1 complex acts via chromatin remodeling and histone modification; it mediates monoubiquitination of histone H2A at Lys-119, resulting in heritable changes in gene expression.
Within the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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