Thermo Fisher Scientific Human Hematopoietic Lineage Antibody Cocktail, eFluor 450, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

20 µL/test

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse

Class

Cocktail

Type

Antibody

Clone

RPA-2.10, OKT3, 61D3, CB16, HIB19, TULY56, HIR2

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

20 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2642588

Product Specific Information

Description: The Human Hematopoietic Lineage eFluor™ 450 Cocktail contains eFluor™ 450-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.

This cocktail contains the following antibodies:

Anti-Human CD2 (RPA-2.10) eFluor™ 450

Anti-Human CD3 (OKT3) eFluor™ 450

Anti-Human CD14 (61D3) eFluor™ 450

Anti-Human CD16 (CB16) eFluor™ 450

Anti-Human CD19 (HIB19) eFluor™ 450

Anti-Human CD56 (TULY56) eFluor™ 450

Anti-Human CD235a (HIR2) eFluor™ 450

Applications Reported: Human Hematopoietic Lineage eFluor™ 450 Cocktail has been reported for use in flow cytometric analysis.

Applications Tested: Human Hematopoietic Lineage eFluor™ 450 Cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Target Information

Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.