Thermo Fisher Scientific CD13 Monoclonal Antibody (WM-15 (WM15)), FITC, eBioscience
인간 CD13 인식 단일클론 항체(WM-15), FITC 결합형으로 흐름세포분석에 최적화되어 있습니다. 5 µL(1 µg)/test 사용, 488 nm/520 nm 형광 특성. 다양한 세포 및 조직에서 CD13 발현 연구에 활용 가능.
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Thermo Fisher Scientific CD13 Monoclonal Antibody (WM-15 (WM15)), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | View 9 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | WM-15 (WM15) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11043278 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The WM-15 monoclonal antibody reacts with human CD13 (aminopeptidase N), a transmembrane ectoenzyme expressed on monocytes and granulocytes, but not lymphocytes. CD13 is also found in endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, and placenta.
CD13 functions include degradation of enkephalins and endorphins, peptide processing, amino acid scavenging, antigen processing, and cell adhesion/migration.
Applications Reported: WM-15 (WM15) antibody has been reported for use in flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometric analysis of lysed whole blood. Use at 5 µL (1 µg) per test (defined as staining a cell sample in a final volume of 100 µL). Cell number may vary from 10⁵ to 10⁸ cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD13 (ANPEP) is a 150–170 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. It catalyzes removal of neutral amino acids from small peptides, activating or inactivating bioactive peptides.
CD13 is involved in extracellular matrix degradation, antigen processing, signal transduction, inflammatory responses, cell motility, and vascularization.
It is expressed by granulocytes, monocytes, myeloid leukemia cells, and is linked to protection against apoptosis and poor carcinoma prognosis. CD13 also serves as an alpha receptor for coronavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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