Thermo Fisher Scientific CD215 (IL-15Ra) Monoclonal Antibody (eBioJM7A4), PE-Cyanine7, eBioscience
인간 IL-15 수용체 알파(IL-15Ra)를 인식하는 마우스 단클론 항체로, PE-Cyanine7 형광 표지. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 고친화도 결합 및 안정적 형광 신호 제공. 4°C 암소 보관, 동결 금지.
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Applications
- Flow Cytometry (Flow): 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioJM7A4 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848316 |
Additional Formats
Product Specific Information
Description:
The eBioJM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Rα). IL-15 and IL-2 share overlapping but distinct biological effects, utilizing common IL-2Rβ and γ chains for signaling. IL-15Rα binds IL-15 with a 1,000-fold higher affinity than IL-2Rα binds IL-2. Three spliced IL-15Rα variants in humans all exhibit high-affinity IL-15 binding. The cytoplasmic domain of IL-15Rα is not required for signaling, indicating its primary role is high-affinity ligand binding. At high concentrations, IL-15 can signal via IL-2Rβ and IL-2Rγ without the α subunit.
Applications Reported:
Suitable for flow cytometric analysis.
Applications Tested:
Pre-diluted and validated for flow cytometric staining of normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test in 100 µL final volume. Cell number per test: 10⁵–10⁸ cells (empirically determined).
Light Sensitivity:
PE-Cyanine7 tandem dye is light-sensitive; protect from exposure.
Fixation:
Samples can be stored in:
- IC Fixation Buffer (Product #00-8222-49)
- 1-step Fix/Lyse Solution (Product #00-5333-57)
Storage: up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation/Emission:
488–561 nm excitation; 775 nm emission.
Compatible lasers: Blue, Green, Yellow-Green.
Target Information
Interleukin 15 (IL-15RA):
Cytokine receptor that binds IL-15 with high affinity, regulating T and NK cell activation and proliferation. Shares IL-2Rβ and IL-2Rγ subunits with IL-2 receptor. IL-15RA binds IL-15 independently and enhances cell proliferation and anti-apoptotic protein expression (Bcl-xL, Bcl-2).
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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