Thermo Fisher Scientific GTF2E1/TFIIE-alpha Polyclonal Antibody
GTF2E1/TFIIE-alpha 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체. Western blot, IHC, IP 등 다양한 응용에 적합. 인간 및 생쥐 반응성, 항원 친화 크로마토그래피로 정제. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 389 and 439 of human General Transcription Factor IIE, polypeptide 1, alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Bovine and Orangutan
Target Information
The general transcription factor TFIIE plays an essential role in eukaryotic transcription initiation with RNA polymerase II and other general factors. Human TFIIE consists of two subunits: TFIIE-alpha (57 kDa) and TFIIE-beta (34 kDa), joining the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is required for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can replace the natural subunit in vitro. Its sequence includes leucine repeat, zinc finger, and helix-turn-helix motifs, showing similarities to bacterial sigma factors, suggesting a regulatory role in transcription initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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