Thermo Fisher Scientific NEDD8 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

10-50 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

10-50 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

10-50 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from the n-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8, Neddylin) conjugated to immunogenic carrier protein has been used as the immunogen if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NEDD8,uniProtId:P29595-1,ncbiNodeId:10090,antigenRange:1,antigenLength:81,antigenImageFileName:OSN00021G-500UG_NEDD8_P29595-1_Sheep.svg,antigenImageFileNamePDP:OSN00021G-500UG_NEDD8_P29595-1_Sheep_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

The antigen is homologous in human, rat, zebrafish, drosophila and xenopus.

Specificity of this antibody: NEDD8.

Target Information

NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.