Thermo Fisher Scientific NEDD8 Polyclonal Antibody
NEDD8 단백질에 특이적인 Sheep Polyclonal Antibody로, WB 및 IHC(F/P) 실험에 적합. 인간, 마우스, 랫트 반응성. PBS 버퍼에 동결건조된 형태로 제공되며, 장기 보관 시 -20°C에서 안정적. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 10–50 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 10–50 µg/mL |
| Immunohistochemistry (Frozen) (IHC-F) | 10–50 µg/mL |
| Miscellaneous PubMed (Misc) | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from the N-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8, Neddylin) conjugated to immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
- The antigen is homologous in human, rat, zebrafish, drosophila, and xenopus.
- Specificity: NEDD8.
Target Information
NEDD8 is a ubiquitin-like protein that plays an important role in cell cycle control and embryogenesis.
Covalent attachment to its substrates requires activation by the E1 complex (UBE1C-APPBP1) and linkage to the E2 enzyme (UBE2M).
Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, promoting polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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