Thermo Fisher Scientific H3R17me2a Polyclonal Antibody
H3R17me2a 다이메틸화 히스톤 H3(Arg17) 인식 폴리클로날 항체로, Western blot, IHC, Dot blot에 적합. 사람 및 마우스 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체. 4°C 단기, -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | — |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | — |
| Dot Blot (DB) | Assay-dependent | — |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acids residues surrounding the di-methyl Arg17 of Histone3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.62 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552255 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with four core histone proteins, it binds to DNA to form the nucleosome structure.
Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability. Post-translational modifications of histones can alter chromatin structure or enable binding of specific transcription factors.
The N-terminal tail of histone H3 can undergo various modifications such as methylation, acetylation, and phosphorylation, influencing cellular processes.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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