Thermo Fisher Scientific Phospho-p38 MAPK (Thr180, Tyr182) Monoclonal Antibody (C.7.8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:200

-

Flow Cytometry (Flow)

1:400-1:1,600

View 1 publication 1 publication

Immunoprecipitation (IP)

1:50

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat, Yeast

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C.7.8

Immunogen

Synthetic phosphopeptide corresponding to residues around pThr180/Tyr182 of human p38 MAPK if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p38 MAPK alpha,uniProtId:Q16539-1,ncbiNodeId:9606,antigenRange:182,antigenLength:360,antigenImageFileName:MA5-15218_p38_MAPK_alpha_Q16539-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15218_p38_MAPK_alpha_Q16539-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

156 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10986063

Product Specific Information

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

It is not recommended to aliquot this antibody.

Target Information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.