Thermo Fisher Scientific Phospho-MAPK15 (Thr175, Tyr177) Polyclonal Antibody
Phospho-MAPK15 (Thr175, Tyr177) 항체로, 인산화된 ERK8 단백질을 특이적으로 검출합니다. Western blot과 면역세포염색에 적합하며, 사람 및 생쥐 시료에 반응합니다. Rabbit polyclonal 형식으로, 1 mg/mL 농도의 액상 시약입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAPK15 (Accession Q8TD08), corresponding to amino acid residues around phosphorylated Thr175 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818068 |
Product Specific Information
This antibody detects endogenous levels of ERK8 only when phosphorylated at Threonine 175 and Tyrosine 177.
Target Information
ERKs are a subfamily of MAPKs involved in cell growth and differentiation. Extracellular signal-regulated kinase 8 (ERK8) is a large MAP kinase whose activity is controlled by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRα by Hic-5 in a kinase-independent manner, suggesting a broader role for ERK8 in regulating nuclear receptors beyond estrogen receptor alpha. Erk8 is also a novel effector of RET/PTC3, contributing to RET biological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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