Thermo Fisher Scientific HACE1 Monoclonal Antibody (OTI10D3), TrueMAB
HACE1 단백질을 인식하는 Mouse IgG2b 단일클론 항체로, Western blot에 적합합니다. 인간, 생쥐, 랫트 반응성이 있으며, 동결건조 형태로 제공됩니다. 고순도 친화 크로마토그래피 정제, 연구용으로 사용됩니다.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10D3 |
| Immunogen | Full length human recombinant protein of human HACE1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using carrier-free antibody, perform an additional round of desalting (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
HACE1 is an E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases.
It regulates Golgi membrane dynamics during the cell cycle, mediating ubiquitination during mitotic Golgi disassembly and reassembly.
Specifically interacts with GTP-bound RAC1, promoting ubiquitination and degradation of active RAC1, contributing to host defense.
May also function as a transcription regulator via interaction with RARB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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