Thermo Fisher Scientific BMI-1 Polyclonal Antibody
BMI-1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, ICC/IF, ELISA에 적합합니다. 인간 시료에 반응하며, Affinity Chromatography로 정제되었습니다. PBS/glycerol buffer에 보관되며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 120–326 of human Bmi1 (NP_005171.4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.27 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805446 |
Product Specific Information
- Immunogen sequence:
EDKRIITDDE IISLSIEFFD QNRLDRKVNK DKEKSKEEVN DKRYLRCPAA MTVMHLRKFL RSKMDIPNTF QIDVMYEEEP LKDYYTLMDI AYIYTWRRNG PLPLKYRVRP TCKRMKISHQ RDGLTNAGEL ESDSGSDKAN SPAGGIPSTS SCLPSPSTPV QSPHPQFPHI SSTMNGTSNS PSGNHQSSFA NRPRKSSVNG SSATSSG - Positive Samples: U-937
- Cellular Location: Cytoplasm, Nucleus
Target Information
Component of the Polycomb group (PcG) multiprotein PRC1 complex, which maintains the transcriptionally repressive state of many genes, including Hox genes, throughout development. The PcG PRC1 complex acts via chromatin remodeling and histone modification, mediating monoubiquitination of histone H2A at Lys-119, leading to heritable changes in chromatin expressibility. Within the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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