Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), APC, eBioscience
인간 및 침팬지 CD3e 인식을 위한 SK7 클론 단일클론 항체로, APC 형광체 결합. Flow cytometry에 최적화되어 있으며, 5 µL/test로 사용. T세포 표지 및 분석 연구용으로 적합. 4°C 암소 저장, 동결 금지.
- 카탈로그번호
- 17-0036-4x (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 18 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Chimpanzee, Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SK7 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10597606 |
Available Formats
- APC-eFluor 780
- Brilliant UV 395
- Brilliant UV 496
- Brilliant UV 563
- Brilliant UV 615
- Brilliant UV 661
- Brilliant Violet 650
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 690
- PE
- PE-Cyanine5.5
- PE-Cyanine7
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. CD3e, along with gamma and delta subunits, is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells.
The SK7 and UCHT1 monoclonal antibodies cross-block binding, suggesting recognition of overlapping epitopes, while OKT3 and SK7 recognize distinct epitopes. SK7 also recognizes chimpanzee CD3.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per test (final volume 100 µL, 10⁵–10⁸ cells/test).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD3 subunit complex is crucial for transducing antigen-recognition signals into T cells and regulating TCR complex expression. CD3 subunits (gamma, delta, epsilon, zeta) are members of the immunoglobulin superfamily encoded on chromosome 11. These subunits associate with signal transduction molecules via tyrosine-based motifs.
CD3 is expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells, but not on B or NK cells. Decreased T lymphocyte percentages may occur in autoimmune diseases. Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures.
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