Thermo Fisher Scientific IL-23 p19 Monoclonal Antibody (727753), PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

10 µL/1x10^6 cells

-

Product Specifications

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

727753

Immunogen

E. coli-derived recombinant human IL-23 p19 Arg20-Pro189 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-23 p19,uniProtId:Q9NPF7-1,ncbiNodeId:9606,antigenRange:20-189,antigenLength:189,antigenImageFileName:MA5-23644_IL-23_p19_Q9NPF7-1_House_mouse.svg,antigenImageFileNamePDP:MA5-23644_IL-23_p19_Q9NPF7-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2577111

Product Specific Information

In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) IL-23 heterodimer is observed, less than 10% cross-reactivity with recombinant mouse (rm) IL-23 heterodimer is observed, and no cross-reactivity with recombinant feline IL-23 p19, recombinant canine (rca) IL-23 p19, and recombinant rat IL-23 p19 is observed. In Western blots, no cross-reactivity with rcaIL-23 p19 or rmIL-23 p19 is observed.

Target Information

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.