Thermo Fisher Scientific HIF1-alpha Polyclonal Antibody
HIF1-alpha 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, IP 등 다양한 응용 가능. 인간 및 생쥐 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체로 연구용에 적합.
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- A300286A
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- pk
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Thermo Fisher Scientific HIF1-alpha Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1, alpha subunit |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on sequence identity, predicted to react with Rat, Chicken, and Bovine
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor present in mammalian cells under reduced oxygen tension. HIF-1 consists of alpha and beta subunits belonging to the PAS family of transcription factors. It regulates adaptive responses to hypoxia by activating transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, and VEGF.
HIF1-alpha influences hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. It interacts with p53 under hypoxic conditions, leading to MDM2-mediated ubiquitination and degradation. Induction of NOX4 by HIF1-alpha helps maintain ROS levels after hypoxia. In humans, HIF1A is located on chromosome 14q and its C-terminal binds p300, forming complexes that induce hypoxia-responsive genes such as VEGF.
Hypoxia plays a critical role in diseases like myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and COPD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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