Thermo Fisher Scientific LIM kinase 1 + 2 Polyclonal Antibody
LIM kinase 1과 2를 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. Human, Mouse, Rat에 반응하며, Protein A로 정제된 액상 형태입니다. 세포 형태 및 이동 조절 연구에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human LIM kinase 1 + 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Proteins containing LIM motifs are typically involved in cell fate determination and growth control. LIM kinases (LIMK-1 and LIMK-2) regulate F-actin stabilization and cofilin activity, indicating their role in signaling pathways that control cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest distinct developmental functions. A truncated LIMK-2 form found in adult testis may result from alternative exon initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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