Thermo Fisher Scientific NGAL Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Lipocalin-2/NGAL Gln21-Asn198 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NGAL,uniProtId:P30152-1,ncbiNodeId:10114,antigenRange:21-198,antigenLength:198,antigenImageFileName:PA5-46938_NGAL_P30152-1_Goat.svg,antigenImageFileNamePDP:PA5-46938_NGAL_P30152-1_Goat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609008

Product Specific Information

This antibody detects rat Lipocalin-2/NGAL in ELISAs and rat and mouse Lipocalin-2/NGAL in Western blots. In sandwich ELISAs, less than 0.4% cross-reactivity with recombinant mouse (rm) Lipocalin-2/NGAL-2 is observed and less than 0.2% cross-reactivity with recombinant human (rh) Lipocalin-1/NGAL-1, rhLipocalin-2/NGAL-2 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.