Thermo Fisher Scientific U2AF65 Polyclonal Antibody
Rabbit 폴리클로날 U2AF65 항체로 Western blot, IHC, IP 등 다양한 응용에 적합. Human U2AF2 단백질의 380–430번 잔기 영역을 면역원으로 제작. 액상 형태, 1 mg/mL 농도, 4°C 보관. FFPE 조직의 IHC 시 citrate buffer pH 6.0 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 380 and 430 of Human U2 Small Nuclear Ribonucleoprotein Auxiliary Factor (65kD) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site.
This gene encodes the U2AF large subunit, which contains a sequence-specific RNA-binding region with three RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing.
The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly.
Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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