
Thermo Fisher Scientific U2AF65 Polyclonal Antibody
Thermo Fisher Scientific의 U2AF65 Polyclonal Antibody는 인간 U2AF65 단백질을 인식하는 토끼 IgG 항체로, Western blot, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 430 and 475 of Human U2 Small Nuclear Ribonucleoprotein Auxiliary Factor (65kD) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, antibody predicted to react with Mouse
Target Information
U2 auxiliary factor (U2AF), composed of large and small subunits, is a non-snRNP protein required for U2 snRNP binding to the pre-mRNA branch site. The large subunit (U2AF65) contains RNA recognition motifs and an Arg/Ser-rich domain essential for splicing. It binds to the polypyrimidine tract of introns early in spliceosome assembly. Multiple transcript variants exist, with two full-length forms identified.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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