Thermo Fisher Scientific MTOR Monoclonal Antibody (OTI4A7), TrueMAB
Human mTOR 단백질(aa 1766–2144)을 항원으로 제작된 Mouse IgG1 단클론 항체. Western blot 및 IHC(P) 실험에 적합하며, 무보존제·동결건조 형태로 제공. 세포 성장 및 암 연구용으로 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: Assay-Dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4A7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1766–2144 of human MTOR produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation.
Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer.
FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics.
Mutations affecting the gene result in Smith-Kingsmore syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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