Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 405

Applications

Tested Dilution

Immunocytochemistry (ICC/IF)

1-10 µg/mL

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ Plus 405 Alexa Fluor™ Plus 405 Alexa Fluor™ Plus 405

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Donkey/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Donkey/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Donkey/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Donkey/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Donkey/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Donkey/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Donkey/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Donkey/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 790/host/Donkey/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 488/host/Donkey/)
• [Alexa Fluor Plus 555 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 555/host/Donkey/)
• [Alexa Fluor Plus 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 594/host/Donkey/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 647/host/Donkey/)
• [Alexa Fluor Plus 680 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 680/host/Donkey/)
• [Alexa Fluor Plus 800 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 800/host/Donkey/)
• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/AP/host/Donkey/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Biotin/host/Donkey/)
• [FITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/FITC/host/Donkey/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/HRP/host/Donkey/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/TRITC/host/Donkey/)
• Request custom conjugation

Excitation/Emission Max

404/455 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 6.5

Contains

0.016% Methylisothiazolone, 0.016% Bromonitrodioxane

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2890547

Target

IgG

Cross Adsorption

against serum proteins from bovine, goat, chicken, guinea pig, hamster, horse, sheep, mouse, rat, and human.

Antibody Form

Whole Antibody

Product Specific Information

To minimize cross-reactivity, the donkey anti-rabbit IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, goat, chicken, guinea pig, hamster, horse, sheep, mouse, rat, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

Specificity: This antibody binds to heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody does not bind non-immunoglobulin rabbit serum proteins or IgG from bovine, goat, chicken, guinea pig, hamster, horse, sheep, mouse, rat, or human.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.