
Thermo Fisher Scientific DPH2 Monoclonal Antibody (OTI3A12), TrueMAB
Human DPH2 단백질을 인식하는 마우스 단일클론 항체로 Western blot, IHC, ICC/IF, Flow Cytometry에 적합. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로 diphthamide 생합성 관련 DPH2 단백질 분석에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A12 |
| Immunogen | Full length human recombinant protein of human DPH2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The DPH2 gene is one of two human genes similar to the yeast dph2 gene, which complements a diphthamide mutant strain and is involved in diphthamide biosynthesis.
Diphthamide is a post-translationally modified histidine residue in elongation factor 2 (EF2), targeted by diphtheria toxin ADP-ribosylation.
Two transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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