Thermo Fisher Scientific Phospho-Rho (Ser188) Polyclonal Antibody
RhoA 단백질의 Ser188 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC(P) 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 세포막 위치의 RhoA 신호전달 연구에 유용합니다.
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Thermo Fisher Scientific Phospho-Rho (Ser188) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the C-terminal epitope KKKSG phosphorylated at Ser188 of human Rho protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human stomach
- Cellular location: Cell membrane
Target Information
RhoA regulates signal transduction pathways linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in microtubule-dependent signaling required for myosin contractile ring formation during cytokinesis and plays an essential role in cleavage furrow formation.
RhoA is required for apical junction formation in keratinocyte cell-cell adhesion and serves as a target for the yopT cysteine peptidase from Yersinia pestis and Yersinia pseudotuberculosis.
It stimulates PKN2 kinase activity and may activate PLCE1. RhoA is activated by ARHGEF2, promoting GDP-GTP exchange, and is essential for SPATA13-mediated regulation of cell migration and adhesion dynamics.
The MEMO1-RHOA-DIAPH1 signaling pathway is crucial for ERBB2-dependent microtubule stabilization at the cell cortex, controlling localization of APC and CLASP2 through GSK3B regulation. Membrane-bound APC enables MACF1 localization necessary for microtubule capture and stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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