Thermo Fisher Scientific POLE2 Polyclonal Antibody
POLE2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(Paraffin) 실험에 적합합니다. 인간 및 마우스 시료에 반응하며, 고순도의 친화 크로마토그래피 정제 항체입니다. -20°C에서 보관하며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human POLE2 (Accession P56282), corresponding to amino acid residues L477–F527 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2853121 |
Product Specific Information
Antibody detects endogenous levels of total POLE2.
Target Information
DNA replication, recombination, and repair are essential for maintaining genome stability and require exonucleases. During DNA replication, these enzymes process Okazaki fragments, while in repair they remove damaged DNA and correct mismatches. DNA polymerases δ and ε are key exonucleases in these processes. DNA polymerase δ has subunits p125 and p50, with p125 interacting with PCNA, and is regulated by cell cycle proteins. DNA polymerase ε is a multisubunit enzyme whose catalytic subunit is encoded by the POL2 gene. The precise roles of DNA polymerases δ and ε in leading and lagging strand synthesis remain under investigation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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