Thermo Fisher Scientific CD45RA Monoclonal Antibody (HI100), PE-Cyanine5, eBioscience
인간 CD45RA를 인식하는 HI100 클론 단일클론 항체로, PE-Cyanine5 형광 표지되어 있습니다. Flow Cytometry용으로 최적화되어 있으며, 5 µL/test로 사용됩니다. 빛에 민감하므로 암조건 보관이 필요하며, 다양한 형광체 포맷으로 제공됩니다.
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Thermo Fisher Scientific CD45RA Monoclonal Antibody (HI100), PE-Cyanine5, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.125 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HI100 |
| Conjugate | PE-Cyanine5 |
| Excitation/Emission Max | 568/666 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10547282 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- Alexa Fluor Plus 555
- Alexa Fluor Plus 750
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 661, 737
- Brilliant Violet 421, 786
- eFluor 450, 506
- FITC
- NovaFluor Blue 660-120S, 800
- NovaFluor Red 710
- NovaFluor UV 765
- NovaFluor Violet 690, 800
- NovaFluor Yellow 610, 660, 810
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 702
- Request custom conjugation
Product Specific Information
The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subsets of CD4+ and CD8+ peripheral T lymphocytes and B cells. CD45RA+ T cells are mainly naive/virgin, making HI100 useful as a phenotypic marker to discriminate T cell subsets.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells. Use at 5 µL (0.125 µg) per test (100 µL final volume).
- Light Sensitivity: Tandem dye is photo-sensitive; protect from light.
- Fixation: Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
- Excitation/Emission: 488–561 nm / 667 nm; compatible with Blue, Green, and Yellow-Green lasers.
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD45RA is an isoform of the CD45 complex with restricted expression among lymphoid cell subtypes. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells and regulates Src-family kinases, lymphocyte development, and antigen signaling. It exists in multiple isoforms due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions. CD45 is vital for signal transduction and immunological regulation. Dysfunction is associated with severe combined immunodeficiency and Hepatitis C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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