Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• Biotin
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily expressed on activated B cells, macrophages, dendritic cells, and activated T cells.
CD80 binds to CD28 and CTLA-4 (CD152) on T cells, playing a crucial role in T-B cell communication and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on Raji cells at 5 µL (1.0 µg) per test
• Recommended Cell Count: 10⁵–10⁸ cells/test
• Excitation/Emission: 350/395 nm
• Laser: Ultraviolet (355 nm)

Note: When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecules CD28 and CTLA-4 (CD152).
Both are expressed on professional antigen-presenting cells and are essential for T cell activation.
CD80 is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cells, follicular B lymphoma cells, T cells, and monocytes.
It provides regulatory signals for T lymphocytes via interaction with CD28 and CTLA4.

Associated Diseases: Gallbladder squamous cell carcinoma, myocarditis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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