Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), Brilliant Ultra Violet 395, eBioscience
인간 CD80(B7-1)을 인식하는 단클론 항체로, Flow Cytometry용으로 검증됨. Brilliant Ultra Violet 395 형광체 결합으로 UV 레이저(355 nm) 기반 분석에 적합. 활성화된 B세포, 대식세포, 수지상세포 표지에 유용. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (1.0 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Ultra Violet™ 395, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D10.4 |
| Conjugate | Brilliant Ultra Violet™ 395 |
| Excitation/Emission Max | 347/399 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, Do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925273 |
Additional Formats Available
- Unconjugated
- APC
- Biotin
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 711
- FITC
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily expressed on activated B cells, macrophages, dendritic cells, and activated T cells.
CD80 binds to CD28 and CTLA-4 (CD152) on T cells, playing a crucial role in T-B cell communication and activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and tested on Raji cells at 5 µL (1.0 µg) per test
- Recommended Cell Count: 10⁵–10⁸ cells/test
- Excitation/Emission: 350/395 nm
- Laser: Ultraviolet (355 nm)
Note: When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecules CD28 and CTLA-4 (CD152).
Both are expressed on professional antigen-presenting cells and are essential for T cell activation.
CD80 is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cells, follicular B lymphoma cells, T cells, and monocytes.
It provides regulatory signals for T lymphocytes via interaction with CD28 and CTLA4.
Associated Diseases: Gallbladder squamous cell carcinoma, myocarditis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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