Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), PerCP-eFluor 710, eBioscience
마우스 IL-4를 특이적으로 인식하는 중화형 단클론 항체로, PerCP-eFluor 710으로 표지되어 유세포분석에 적합합니다. Th2 세포 유래 IL-4 검출에 사용되며, 488 nm 레이저로 여기되어 710 nm에서 방출됩니다. 실험 조건에 따라 최적화된 희석으로 사용 가능합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), PerCP-eFluor 710, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | ≤ 0.125 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11B11 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573826 |
Available Formats:
- Unconjugated
- Alexa Fluor 488
- APC
- Brilliant UV 615
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 786
- Functional Grade
- PE
- PE-Cyanine7
- Custom conjugation available
Product Specific Information
The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine, and functions as a neutralizing antibody. Mouse IL-4 (also known as BCDF, BCGF-1, BSF-1) is secreted primarily by activated Th2 and NKT cells, and to a lesser extent by Th1 and mast cells. IL-4 promotes B cell proliferation, differentiation, and Ig isotype switching, and upregulates surface MHC class II and CD23.
Applications Reported:
Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining and flow cytometry of Th2-polarized, stimulated mouse splenocytes. Recommended use ≤ 0.125 µg/test (defined as staining a 100 µL cell sample). Cell number should be empirically determined (10⁵–10⁸ cells/test). Titration is recommended for optimal performance.
Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Recommended filter: 710/50 bandpass (695/40 acceptable). Ensure instrument compatibility.
Fixation:
Samples can be stored in:
- IC Fixation Buffer (Product #00-8222-49)
- 1-step Fix/Lyse Solution (Product #00-5333-54)
Storage up to 3 days at 4°C in the dark with minimal signal loss. Clone-specific fixation performance should be empirically verified.
Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Interleukin-4 (IL-4) is a pleiotropic cytokine produced by activated T cells and acts as a ligand for the IL-4 receptor. It shares receptor components with IL-13, leading to overlapping biological functions. STAT6 is a key mediator of IL-4 signaling. The IL-4 gene is part of a cytokine cluster on chromosome 5q, closely located to IL-13 and IL-5, regulated by long-range genomic elements. IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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