Thermo Fisher Scientific ERK1 Monoclonal Antibody (1E5)
ERK1(MAPK3)을 표적하는 Thermo Fisher Scientific의 단클론 항체로, WB, IHC, ICC, Flow, ELISA에 적합합니다. 인간, 생쥐, 원숭이, 랫트 시료에 반응하며, 세포 신호전달 연구에 활용됩니다. 냉장 또는 냉동 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E5 |
| Immunogen | Purified recombinant fragment of human MAPK3 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C for short term. For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11151830 |
Product Specific Information
MA5-15896 targets MAPK3 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with human, mouse, non-human primate, and rat samples.
The immunogen is a purified recombinant fragment of human MAPK3 expressed in E. coli.
MA5-15896 detects MAPK3, which has a predicted molecular weight of approximately 44 kDa.
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that acts as an important activator of p90, RSK, MSK, ELK1, and Stat3, and plays a key role in the MAPK pathway.
ERK1 is widely expressed and regulates meiosis, mitosis, and post-mitotic functions in differentiated cells.
It is activated by various stimuli such as growth factors, cytokines, viral infection, GPCR ligands, and transforming agents.
Functionally, ERK1 participates in signaling cascades controlling proliferation, differentiation, and cell cycle progression in response to extracellular signals.
Upon activation, ERK1 translocates to the nucleus to phosphorylate nuclear targets.
Alternatively spliced transcript variants encoding different isoforms of ERK1 have been identified.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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